Isolation and Expression of HAP2, A Homolog of AP2 in Hyacinthus orientalis L.
LI Quan-zi, LI Xing-guo and ZHANG Xian-sheng* College of Life Sciences, Shandong Agricultural University, Taian 271018, China BAI Shu-nong* * College of Life Sciences, Peking University, Beijing 100871, China LU Wen-liang** ** Institute of Botany, Chinese
By precisely controlling exogenous hormones applied in the cultural process, particular type of floral organs, such as tapels, stamens, or ovules, can be regenerated from perianth explant of Hyacinthus orientalis L. To further study the molecular mechanism of the specific organgenesis in the system, an AP2 homolog, HAP2 was isolated from regenerated tepals by using RT-PCR. Results from RT-PCR combined with Southern hybridization showed that HAP2 was expressed in leaves, perianth, regenerated tepals and regenerated stamens. The possible functions of HAP2 on hormone-regulated floral organ regeneration are discussed.