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《Acta Laser Biology Sinica》 2017-04
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Targeting and Long Circulating Drug Delivery System Base on Mannose-conjugated PEG-modified Nano-liposomes for Tumor Chemotherapy

YANG Fan;QIN Aiping;LI Jing;PENG Qian;WANG Chenxu;HONG Xiuqin;Institute of Geriatric,People's Hospital of Hunan Province( the First Affiliated Hospital of Hunan Normal University);  
Genistein doped mannose-conjugated PEG-modified liposomes( man-PEG@ LP/Ge) were prepared via filmsonic method by using targeted lipid DSPE-PEG-man and helper lipid DOTAP. Concanavalin A( Con A) agglutination assay had proved that mannose has been modified to nano-liposomes successfully. The as-prepared man-PEG @ LP/Ge were spherical in shape with a homogeneous size distribution around( 270 ± 15) nm in diameter as characterized by SEM. The Zeta potential was + 7. 5 m V in pure water. Furthermore,man-PEG@ LP/Ge appeared to be very homogeneous and stable in water. It is considered that the steric-hinerance effects of surface PEG long chain and the strong electrostatic repulsion of the positive charge play an important role in the good dispersion of man-PEG@ LP/Ge. Flow cytometry examination confirmed the targeting specificity of man-PEG@ LP/Ge against human hepatoma Huh-7 cells which are of over-expression of mannose receptors. The cell toxicity viability demonstrated that unloaded man-PEG@ LP are practically non-toxic,indicating that the drug delivery vehicles possess excellent biocompatibility. Systematic in vitro and in vivo studies showed that the man-PEG @ LP/Ge kill tumor cells with high specificity and excellent efficiency,owing to the mannose-mediated active tumor-targeted drug delivery.
【Fund】: 湖南省科技厅重点研发计划项目(2015JC3117)
【CateGory Index】: R73-36
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