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《Fudan University Journal of Medical Sciences》 2015-01
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Neuroprotective effects of flavonoids extracted from the leaves of Diospyros kaki in D-galactose induced aging mice by attenuating oxidative stress and neuroinflammation

MA Ying-juan;SHANG Yu-ying;WANG De-jie;SUN Meng-han;HOU Xun-Yao;HONG Yan;ZENG Zi-ling;LIU Xue-ping;Department of Senile Neurology,Provincial Hospital Affiliated to Shandong University;Department of Epidemiology and Statistics,Medical College,Qingdao University;  
Objective To investigate the effects of flavonoids extracted from the leaves of Diospyros kaki on praxiology and the possible mechanisms involving oxidative stress and inflammation of the brain in aging mice induced by D-galactose. Methods Forty Kunming male mice were randomly divided into four groups:normal control group(NC group),model group(D-gal group),low-dose flavonoids intervention group(D-gal+F40group)and large-dose flavonoids intervention group(D-gal+F 80group).D-gal group and two intervention groups received injection of D-galactose 100mg·kg-1·d-1for 60 days to build aging model.D-gal+F40group and D-gal+F 80 group were fed with flavonoids 40 and 80mg·kg-1·d-1 respectively by oral gavage,while NC group and D-gal group were fed with the same volume of normal saline.Morris water maze was used for testing escape latency(EL)and the number of crossing,related kits were used for detecting superoxide dismutase(SOD)activity,glutathione peroxidase(GSH-Px)activity and malondialdehyde(MDA)content in the brain tissue,enzyme-linked immunosorbent assay(ELISA)and immunohistochemistry were used for detecting the expression of inflammation factors,tumor necrosis factor-alpha(TNF-α)and interleukin-1beta(IL-1β). Results Compared with NC group,EL elevated,the number of crossings decreased,oxidative stress and inflammatory cytokines levels increased significantly in D-galactose induced aging mice.Compared with D-gal group,flavonoids extracted from Diospyros kaki obviously decreased EL,increased the number of crossings,increased SOD,GSH-Px activity and decreased MDA content,reduced the expression of TNF-αand IL-1βin D-gal+F40group and D-gal+F80group. Conclusions Flavonoids from leaves of Diospyros kaki could obviously improve cognitive function in subacute aging mice induced by D-galactose.It also improved the antioxidant ability,lowered inflammation levels in the brain with the function of delaying brain aging.
【Fund】: 国家自然科学基金(81371225)~~
【CateGory Index】: R285.5
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